论文部分内容阅读
目的探讨缺氧诱导因子1α(HIF-1α)mRNA与葡萄糖转运蛋白1(Glut1)mRNA在肝细胞癌(HCC)中的表达及临床意义,并分析两者的关系。方法采用RT-PCR的方法对21例新鲜肝癌样本和10例正常肝组织样本中Glut1mRNA与HIF-1αmRNA的表达情况进行检测。结果 HIF-1αmRNA和Glut1mRNA在HCC患者样本中的阳性表达率明显高于正常肝组织样本(P<0.05);在HCC患者中HIF-1αmRNA和Glut1mRNA的表达均与肿瘤大小、病理分级、临床分期、有无淋巴结转移相关(P<0.05);两者的表达呈正相关(r=0.652,P<0.05)。结论 HIF-1αmRNA和Glut1mRNA过表达与HCC患者的临床特征密切相关,两者的表达呈正相关。
Objective To investigate the expression and clinical significance of hypoxia inducible factor 1α (HIF-1α) mRNA and glucose transporter 1 (Glut1) mRNA in hepatocellular carcinoma (HCC) and to analyze their relationship. Methods The expressions of Glut1 mRNA and HIF-1α mRNA in 21 fresh hepatocarcinoma samples and 10 normal liver tissue samples were detected by RT-PCR. Results The positive rates of HIF-1αmRNA and Glut1mRNA in HCC patients were significantly higher than those in normal liver tissues (P <0.05). The expression of HIF-1αmRNA and Glut1mRNA in HCC patients was significantly correlated with tumor size, pathological grade, clinical stage, (P <0.05). There was a positive correlation between them (r = 0.652, P <0.05). Conclusion The overexpression of HIF-1αmRNA and Glut1mRNA is closely related to the clinical features of HCC patients, and the expression of HIF-1αmRNA and Glut1mRNA is positively correlated.