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用抗Ｃ－ｅｒｂＢ－２癌基因产物Ｐ１８５多克隆抗体２１Ｎ，应用免疫组化链菌素亲生物素蛋白－过氧化酶（Ｓ－Ｐ）方法，对６９例良恶性鼻咽组织及人鼻咽癌细胞系ＣＮＥ－１、ＣＮＥ－２Ｚ、克隆株ＣＮＥ－２Ｚ－Ｆ１、Ｈ５、Ｂ７中Ｐ１８５的表达进行了检测；此外，还观察了克隆株ＣＮＥ－２Ｚ－Ｂ７在连续传代过程中Ｐ１８５的表达。结果显示：鼻咽癌组织中Ｐ１８５表达率为６６．７％，慢性鼻咽炎中Ｐ１８５阳性表达率为６．３％，人胚鼻咽上皮不表达Ｐ１８５；鼻咽癌细胞系及克隆株中Ｐ１８５表达不一；克隆株ＣＮＥ－２Ｚ－Ｂ７在连续传代过程中Ｐ１８５的表达逐渐升高。结论：Ｃ－ｅｒｂＢ－２癌基因的激活，可能在鼻咽癌发生中起重要的作用；Ｐ１８５的过度表达和肿瘤细胞的分化程度及演进过程有关。
Using anti-C-erbB-2 oncogene P185 polyclonal antibody 21N, immunohistochemical streptavidin-peroxidase (S-P) method, 69 cases of benign and malignant nasopharyngeal tissues and human nasopharyngeal The expression of P185 in cancer cell lines CNE-1, CNE-2Z and CNE-2Z-F1, H5 and B7 were detected. In addition, the expression of P185 in the cloned CNE-2Z-B7 . The results showed that the expression of P185 in nasopharyngeal carcinoma was 66.7%, P185 in chronic nasopharyngitis was 6.3%, P185 was not expressed in human nasopharyngeal epithelium, P185 The expression of P185 in the cloned CNE-2Z-B7 was gradually increased during the continuous passage. Conclusion: The activation of C-erbB-2 oncogene may play an important role in the carcinogenesis of nasopharyngeal carcinoma. The overexpression of P185 is related to the differentiation degree of tumor cells and the evolution process.