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建立了 6 巯基嘌呤、硫唑嘌呤和 8 氮杂鸟嘌呤 3种抗癌嘌呤化合物的滤纸基质室温光分析新方法。详细探讨了影响其室温光发射的各种因素。该方法的线性范围、检出限、精密度分别为 6 巯基嘌呤2 0～ 4 .0× 10 2 μmol/L ,1.3μmol/L ,4 .1% ;硫唑嘌呤 2 .0～ 6 .0× 10 2 μmol/L、1.2 μmol/L、3.8% ;8 氮杂鸟嘌呤1.1～ 2 .2× 10 2 μmol/L、7.4× 10 -2 μmol/L、1.3%。用该方法进行尿液中嘌呤化合物的回收测定 ,回收率为 96 .2 %～ 10 1.7% ;进行 6 MP在药片中的回收测定 ,回收率为 96 .2 %～ 10 2 .1%。
A new method was established for the analysis of filter paper substrate room temperature by 6th mercaptopurine, azathioprine and 8-azaguanine. The various factors affecting the room temperature light emission are discussed in detail. The linear range, the detection limit and the precision of this method are respectively: 6-mercaptopurine 2.0-4.0 × 10 2 μmol / L, 1.3 μmol / L, 4.1%; azathioprine 2.0-0.6.0 × 10 2 μmol / L, 1.2 μmol / L, 3.8%, 8-azaguanine 1.1 ~ 2.2 × 10 2 μmol / L and 7.4 × 10 -2 μmol / L, 1.3% respectively. The recovery of purine compounds in urine was measured by this method. The recoveries ranged from 96.2% to 10 1.7%. The recovery of 6 MP in tablets was determined and the recovery was 96.2% -102.1%.