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AIM:To explore the effects of mifepristone,a progesteronereceptor (PR) antagonist,on the proliferation of human gastricadenocarcinoma cell line SGC-7 901 in vitro and the possiblemechanisms involved.METHODS:In situ hybridization was used to detect theexpression of PR mRNA in SGC-7 901 cells.After treatmentwith various concentrations of mifepristone (2.5,5,10,20 μmol/L) at various time intervals,the ultrastructuralchanges,cell proliferation,cell-cycle phase distribution,andthe expression of caspase-3 and Bcl-X_L were analyzed usingtransmission electron microscopy (TEM),tetrazolium blue(MTT) assay,~3H-TdR incorporation,flow cytometry,andreverse transcription-polymerase chain reaction (RT-PCR).RESULTS:Mifepristone markedly induced apoptosis andinhibited cell proliferation of PR- positive SGC-7 901 cellsrevealed by TEM,MTr assay and ~3H-TdR incorporation,ina dose- and time-dependent manner.The inhibitory ratewas increased from 8.98% to 51.29%.Flow cytometricanalysis showed mifepristone dose-dependently decreasedcells in S and G_2/M phases,increased cells in G_0/G_1 phase,reduced the proliferative index from 57.75% to 22.83%.In addition,mifepristone up-regulated the expression ofcaspase-3,and down- regulated the Bcl-X_L expression,dose-dependently.CONCLUSION:Mifepristone effectively inhibited theproliferation of PR-positive human gastric adenocarcinoma cellline SGC-7 901 in vitro through multiple mechanisms,and maybe a beneficial agent against human adenocarcinoma.
AIM: To explore the effects of mifepristone, a progesterone receptor (PR) antagonist, on the proliferation of human gastric adenocarcinoma cell line SGC-7 901 in vitro and the possible mechanisms involved. METHODS: In situ hybridization was used to detect the expression of PR mRNA in SGC -7 901 cells. After treatment with various concentrations of mifepristone (2.5, 5, 10, 20 μmol / L) at various time intervals, the ultrastructuralchanges, cell proliferation, cell-cyclephase distribution, and the expression of caspase-3 and Bcl-X_L were analyzed using transmission electron microscopy (TEM), tetrazolium blue (MTT) assay, ~ 3H-TdR incorporation, flow cytometry, and reverse transcription-polymerase chain reaction SGC-7 901 cells werevealed by TEM, MTr assay and ~ 3H-TdR incorporation, ina dose- and time-dependent manner. The inhibitory rate was increased from 8.98% to 51.29%. Flow cytometric analysis showed mifepristone do se-dependently decreased cells in S and G_2 / M phases, increased cells in G_0 / G_1 phase, reduced the proliferative index from 57.75% to 22.83%. Addition, mifepristone up-regulated the expression of caspase-3, and down- regulated the Bcl -X_L expression, dose-dependently. CONCLUSION: Mifepristone substantially inhibited the proliferation of PR-positive human gastric adenocarcinoma cellline SGC-7 901 in vitro through multiple mechanisms, and maybe a beneficial agent against human adenocarcinoma.