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油菜素内酯(brassinosteroids,BRs)对水稻株高、叶夹角等重要农艺性状具有明显的调控作用,鉴定水稻中受BR调控的蛋白质对揭示BR调控水稻生长发育特定生化过程的潜在机制具有重要的意义。该研究以日本晴水稻(Oryza sativa L.ssp.japanica cv.Nipponbare)为研究材料,用明显影响水稻地上部生长的BR浓度处理,提取地上部膜蛋白,经双向电泳及质谱分析,鉴定到7个受BR调控的蛋白质:光系统II稳定因子HCF136、PMRP(putative membrane related protein,gi|113565516)、ATP synthase(gi|113611230)、gi|113594641、gi|22831029、gi|47497322和gi|56784135,对这些蛋白的功能鉴定可以为阐明BR调控水稻生长发育的机理提供新的思路和途径。其中功能未知的膜蛋白PMRP受BR下调,经亚细胞定位,PMRP定位在细胞质膜上,经生物信息学分析,PMRP具有磷脂酰胆碱结合位点,可能影响膜的组分进而参与水稻抗逆性调控;PMRP RNAi转基因拟南芥对冷害的抵抗能力增强,说明BR可通过调控PMRP的表达提高植物的抗冷性。
Brassinosteroids (BRs) play an important role in controlling plant height, leaf angle and other important agronomic traits. Identification of BR-regulated proteins in rice plays an important role in revealing the underlying mechanism by which BR regulates specific biochemical processes in rice growth and development Meaning. In this study, Oryza sativa L.ssp.japanica cv. Nipponbare was used as research material, and the above-ground membrane protein was extracted with BR concentration that significantly affected the shoot growth of rice. Two-dimensional gel electrophoresis and mass spectrometry analysis identified seven BR-regulated proteins: light system II stabilizing factor HCF136, putative membrane related protein (gi | 113565516), ATP synthase (gi | 113611230), gi | 113594641, gi | 22831029, gi | 47497322 and gi | 56784135, The functional identification of these proteins can provide new ideas and ways for elucidating the mechanism by which BR regulates the growth and development of rice. Among them, PMRP, a membrane protein with unknown function, is down-regulated by BR. Through subcellular localization, PMRP is located on the plasma membrane. Through bioinformatics analysis, PMRP has a phosphatidylcholine binding site, which may affect membrane components and then participate in rice resistance Sexual regulation; PMRP RNAi transgenic Arabidopsis resistance to chilling injury increased, indicating that BR can regulate the expression of PMRP to improve plant cold resistance.