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以综合性状优良的烤烟种质Y3为轮回亲本,抗烟草黑胫病(0号和1号生理小种)和赤星病的雪茄烟种质Beinhart 1000-1及优质烤烟品种K326为供体亲本,连续回交并结合分子标记辅助选择,构建国内外第一套由256个具有烤烟Y3遗传背景并渐渗有Beinhart 1000-1和K326染色体片段株系代换系群体。该群体携带377个代换片段,分布于烟草24条连锁群上。每个株系携带1~5个代换片段,代换片段长度介于0.05~36.88 c M,平均长度7.75 c M。代换片段重叠累加总长度为2922.57 c M,是烟草基因组总长度的2.61倍。代换片段覆盖总长度为1114.32 c M,烟草基因组覆盖率为99.45%。本研究构建的片段代换系可用于烟草基因定位、复杂性状的QTL分析和标记辅助选择育种。
Tobacco black shank (Physiological race 0 and 1) and Beinhart 1000-1 and K326, a good-quality flue-cured tobacco, were used as donor parents with good traits of Y3 as the recurrent parent, A total of 256 substitution lines with Beinhart 1000-1 and K326 chromosomal segments were constructed by 256 backcrossing combined with molecular marker-assisted selection. The population carried 377 substitution fragments distributed in 24 linkage groups of tobacco. Each strain carries 1 to 5 substitution fragments, and the length of the substitution fragment ranges from 0.05 to 36.88 cM with an average length of 7.75 cM. The overlapping length of the substitution fragment was 2922.57 cM, which was 2.61 times of the total length of tobacco genome. Substitute fragments covered a total length of 1114.32 cM, tobacco genome coverage was 99.45%. The segmented substitution lines constructed in this study can be used for tobacco gene mapping, QTL analysis of complex traits and marker-assisted selection breeding.