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目的:建立RP-HPLC测定头孢克肟口含片中头孢克肟含量的方法。方法:色谱柱为ReliaSil C_18(250 mm×4．6 mm, 5μm),流动相为四丁基氢氧化铵溶液(用1．5 mol·L~(-1)磷酸溶液调节pH至7．0)-乙腈(70:30),检测波长254nm。流速0．8 ml·min~(-1)。结果:头孢克肟浓度在2-120μg·ml~(-1)范围内,与峰面积呈良好的线性关系,r=0．999 9;平均回收率为100．6％(n= 9),RSD=1．4％。结论:该方法简便可靠,可用于头孢克肟口含片的质量控制。
Objective: To establish a method for the determination of cefixime in cefixime oral tablets by RP-HPLC. Methods: The column was ReliaSil C18 (250 mm × 4.6 mm, 5 μm) with tetrabutylammonium hydroxide solution (adjusted to pH 7.0 with 1.5 mol·L -1 phosphoric acid solution) Acetonitrile (70:30), detection wavelength 254nm. The flow rate was 0.8 ml · min ~ (-1). Results: The concentration of cefixime in the range of 2-120μg · ml ~ (-1) showed a good linear relationship with the peak area, r = 0.999 9; the average recovery was 100.6% (n = 9) RSD = 1.4%. Conclusion: The method is simple and reliable and can be used for the quality control of cefixime oral buccal tablets.