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目的:研究大豆异黄酮对氧化鱼油造成仔猪氧化应激的缓解作用,并初步探讨其作用机制。创新点:首次在仔猪肠道氧化应激模型中证明大豆异黄酮可缓解仔猪氧化应激,且此作用与提高其免疫功能相关。方法:将72头平均体重约1.8 kg的4日龄杜×(长×大)三元杂公猪随机分为3组,每组重复6次,每次重复包括4头猪:对照组(饲喂50 g/kg新鲜鱼油),处理组1(添加50 g/kg氧化鱼油)和处理组2(添加50 g/kg氧化鱼油+20 mg/kg大豆异黄酮);基础日粮根据NRC(2012)《猪的营养需要量》配制,每3小时饲喂一次,记录每天采食量;实验期21天结束后,称重,计算平均日采食量、平均日增重及料重比。用苏木精-伊红染色法观察小肠形态结构,并计算绒毛高度和隐窝深度,应用南京建成试剂盒测定肠道中抗氧化酶的活性,应用酶联免疫吸附测定(ELISA)试剂盒测定细胞炎症相关因子和细胞凋亡相关因子的表达。结论:饲喂氧化鱼油引起新生仔猪肠道产生氧化应激,引起仔猪生长性能下降、肠道发育受损、细胞凋亡增加及免疫功能失调。而添加大豆异黄酮能减缓氧化鱼油造成的肠道损伤,提高肠道粘膜完整性,提高肠道粘膜细胞抗氧化功能和免疫功能。
Objective: To study the effect of soy isoflavones on oxidative stress in piglets induced by oxidized fish oil, and to explore its mechanism. Innovative point: the first time in piglet intestine oxidative stress model proved that soy isoflavones can alleviate oxidative stress in piglets, and this effect and improve their immune function. Methods: Seventy-two 4-day-old Du × (L × L) ternary boars with an average body weight of about 1.8 kg were randomly divided into three groups with 6 replicates each including 4 pigs each: control group (50 g / kg of oxidized fish oil) and treatment group 2 (of 50 g / kg of oxidized fish oil + 20 mg / kg of soy isoflavones); basal diet according to NRC (2012 ) “Pig nutritional requirements” preparation, every 3 hours feeding, record daily feed intake; experimental period after 21 days, weighing, calculate the average daily feed intake, the average daily gain and feed to weight ratio. The morphology of the small intestine was observed by hematoxylin-eosin staining and the villus height and crypt depth were calculated. The anti-oxidative enzyme activity in the intestine was determined by Nanjing built kit. The cells were determined by enzyme-linked immunosorbent assay (ELISA) Inflammation-related factors and expression of apoptosis-related factors. CONCLUSION: Oxidation of fish oil causes oxidative stress in the gut of newborn piglets, resulting in decreased growth performance, impaired intestinal development, increased apoptosis and immune dysfunction. The addition of soy isoflavones can slow the intestinal damage caused by oxidation of fish oil, improve intestinal mucosal integrity, improve intestinal mucosal cells antioxidant and immune function.