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本研究采用抗生素筛选种子的方法对126份旱稻297的Ac转基因材料T2代种子做抗性筛选,经T3、T4、T5逐代筛选得到64份纯合材料和23份非纯合材料,共87个转化事件(94个株系)。分析了后代株系与产量相关的11个农艺性状变异特点,通过方差分析、相关性分析、主成份分析以及系统聚类分析,结果表明:1)转Ac基因株系间多数农艺性状围绕对照旱稻297均值发生了较大幅度的变异;2)各性状间存在复杂的相关性,尤其株高越高主茎穗总粒数相对较多,主茎结实率较大,千粒重相对较大,是可用作提高产量潜力的有利相关变异;3)11个农艺性状的大部分信息可以由前6个主成份解释,主成份1和主成份2累积贡献率近50%,显示转基因优良株系应选株高较高、主茎穗总粒数较多、主茎穗结实率较高、粒长宽比较大、而且单株有效穗数较少、粒宽和粒厚较小的变异类型;4)聚类分析表明,全部转Ac株系所有农艺性状的变异为6大类型,其中第四、第一两类型的变异更有利于提高产量潜力,并从中选出2个综合性状变异表现突出优良的转Ac株系。这一结果可为转基因水旱稻育种研究的种质创新理论与实践提供借鉴。
In this study, we selected 126 hybrid rice Ac of Acigenotype 292 to screen the seeds of T2 generation by antibiotic screening. After screening by T3, T4 and T5 generations, 64 homozygous materials and 23 non-homozygous materials Transformation event (94 lines). Through analysis of variance, correlation analysis, principal component analysis and cluster analysis, the results showed that: 1) Most of the agronomic characters between Ac-transgenic lines were around the control upland rice 297 mean value occurred more significant variation; 2) there is a complex correlation between the traits, especially the higher the height of the main stem of the total grain number is relatively large, the main stem seed setting rate is relatively large, 3) Most of the 11 agronomic traits can be explained by the first 6 principal components, and the cumulative contribution rate of principal component 1 and principal component 2 is nearly 50%, indicating that the best transgenic lines should be selected Higher plant height, higher total number of main stem spikes, higher rate of seed-setting of main stem, larger grain length-width ratio, smaller number of effective spikes per plant, smaller grain width and grain thickness; 4) Cluster analysis showed that all agronomic traits of Ac-transgenic lines had six major types of variation, of which the fourth and first two types of variation were more conducive to increasing yield potential, and two comprehensive traits were selected to highlight the outstanding variation Turn Ac strain. This result can provide reference for the theory and practice of germplasm innovation of transgenic rice breeding.