论文部分内容阅读
人为诱导大白菜干烧心病发生，通过超速离心制备心叶组织细胞膜微囊，测定各细胞器膜的Ｃａ２＋－ＡＴＰａｓｅ活性变化。结果表明，随缺钙天数增加，细胞膜总Ｃａ２＋－ＡＴＰａｓｅ、ＰＭ－Ｃａ２＋－ＡＴＰａｓｅ和ＴＮ－Ｃａ２＋－ＡＴＰａｓｅ活性迅速上升，在处理后第３天达到高峰，随后逐渐下降，而潜在性Ｃａ２＋－ＡＴＰａｓｅ活性却呈逐渐上升趋势。同时，细胞膜透性和丙二醛含量逐渐增大，超氧化物歧化酶（ＳＯＤ）和过氧化物酶（ＰＯＤ）活性则逐渐下降。推测该病的发生与细胞膜钙泵活性变化诱导的钙的第二信使功能有关。
Artificial induction of Chinese cabbage dry heart disease occurred by ultracentrifugation of the heart cell membrane microcapsules, determination of the organelle membrane Ca2 + -ATPase activity changes. The results showed that the activities of total Ca2 + -ATPase, PM-Ca2 + -ATPase and TN-Ca2 + -ATPase increased rapidly with the increase of Ca2 + -added days, reached its peak on the third day after treatment and then decreased gradually, while the potential Ca2 + -ATPase activity But showed a gradual upward trend. At the same time, cell membrane permeability and malondialdehyde content gradually increased, and the activities of superoxide dismutase (SOD) and peroxidase (POD) decreased gradually. It is speculated that the occurrence of the disease is related to the second messenger function of calcium induced by the change of the calcium pump activity of the cell membrane.