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目的建立一种反相高效液相色谱法,同时测定病窦灵口服液中的2种有效成分。方法采用Alltech C18柱,乙腈(A)-0.2%冰醋酸(B)溶液梯度洗脱,流速1.0 mL.min-1,检测波长270 nm,柱温30℃。结果采用HPLC检测制剂中丹酚酸B和淫羊藿苷的含量,其线性关系良好,线性范围分别为:25.81~413.0μg.mL-1,2.97~59.36μg.mL-1,丹酚酸B回收率为99.77%,RSD为0.5%;淫羊藿苷回收率为100.1%,RSD为2.3%。结论本测定方法简便可行、重复性好,可用于制剂的质量控制。
Objective To establish a reversed-phase high performance liquid chromatography (HPLC) method for the simultaneous determination of two active ingredients in Suanualing oral solution. Methods Alltech C18 column was used. The gradient of acetonitrile (A)-0.2% glacial acetic acid (B) was used to elute the solution. The flow rate was 1.0 mL.min-1. The detection wavelength was 270 nm and the column temperature was 30 °C. Results The content of salvianolic acid B and icariin in the preparation was detected by HPLC. The linear relationship was good. The linear ranges were: 25.81~413.0μg.mL-1, 2.97~59.36μg.mL-1, respectively. The recovery rate was 99.77%, RSD was 0.5%, the icariin recovery rate was 100.1%, and the RSD was 2.3%. Conclusion The method is simple, feasible and reproducible. It can be used for the quality control of preparations.