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目的建立五味子提取物的定性鉴别和含量测定标准。方法采用TLC鉴别五味子;采用紫外可见分光光度法,570nm波长,测定五味子木脂素含量;采用RP-HPLC,以甲醇-乙腈-水(1∶1∶1)为流动相,250nm为检测波长,测定五味子醇甲、五味子甲素、五味子乙素含量。结果供试品色谱中,在与对照品和对照药材色谱相应的位置上,显示相同颜色的荧光斑点;五味子木脂素以乙素计,在0.0055～0.0267mg·mL-1内,线性关系良好,平均回收率101.4%(RSD=2.5%);五味子醇甲在0.275～1.375μg(r=0.9997)内、五味子甲素在0.125～0.625μg(r=0.9997)内、五味子乙素在0.160～0.800μg(r=0.9997)内,线性关系良好,平均回收率分别为97.9%(RSD=1.4%)、99.0%(RSD=1.9%)、98.2%(RSD=1.9%)。结论采用方法简便、准确、快速,可对五味子提取物进行有效的质量控制。
Objective To establish a qualitative identification and determination of Schisandra sp. Methods The TLC was used to identify Schisandra chinensis. The content of Schisandra chinensis was determined by UV-Vis spectrophotometry at 570 nm. The mobile phase was methanol-acetonitrile-water (1: 1: 1) Determination of schisandrin A, Schisandra A, Schisandrin B content. The results for the test product chromatography, and the reference substance and reference drug chromatography corresponding position, showing the same color fluorescent spots; Schisandra lignans in terms of B, 0.0055 ~ 0.0267mg · mL-1 within a good linear relationship , And the average recovery was 101.4% (RSD = 2.5%). Schisandrin A was in the range of 0.275-1.375μg (r = 0.9997), Schizandrin A was 0.125-0.625μg (r = 0.9997) (r = 0.9997). The average recoveries were 97.9% (RSD = 1.4%), 99.0% (RSD = 1.9%) and 98.2% (RSD = 1.9%) respectively. Conclusion The method is simple, accurate and rapid, which can effectively control the quality of Schisandra extract.