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目的研究甘肃不同产地黄芩根、茎叶的清热燥湿作用异同。方法按照体重将SD大鼠随机分为空白组、模型组、陇西产黄芩Ⅰ组(根)、陇西产黄芩Ⅱ组(茎叶)、渭源产黄芩Ⅰ组(根)、渭源产黄芩Ⅱ组(茎叶)、漳县产黄芩Ⅰ组(根)、漳县产黄芩Ⅱ组(茎叶)、岷县产黄芩Ⅰ组(根)、岷县产黄芩Ⅱ组(茎叶),共10组。除空白组以外,其余各组大鼠给予肥甘饲料喂养和56度红星二锅头灌胃(5 mL·kg~(-1)),每日1次,并置于人工气候箱中,连续21 d;第22天,将浓度为1×10~9/mL大肠杆菌灌胃1次(10μL·g~(-1));4 h后,加强感染1次(5μL·g~(-1)),建立大鼠湿热证模型。空白组大鼠灌胃0.9%NaCl 10 mL·kg~(-1);各药物组大鼠,在模型制备的同时,分别给予黄芩根或茎叶水煎液10 g·kg~(-1)灌胃,均每天1次,连续7 d。观察各组大鼠证候学表现,以酶联免疫吸附法检测血清中白介素4(IL-4)、干扰素γ(IFN-γ)、胃动素(MTL)、胃泌素(GAS)的含量以及胃肠组织中MTL、GAS的含量。结果与模型组的IL-4、IFN-γ含量分别为(38.09±1.41),(1203.90±32.24)ng·L-1比较,各药物组大鼠血清中IL-4、IFN-γ的含量分别为(25.36±2.56),(703.53±29.36);(31.54.±3.81),(810.90±24.07);(26.88±2.91),(679.02±30.72);(30.81±3.19),(802.44±20.69);(26.61±3.94),(702.90±14.86);(32.45±5.12),(815.36±34.78);(27.06±4.01),(685.66±42.05);(31.76±3.98),(808.95±38.22)ng·L~(-1),差异均有统计学意义(均P<0.05)。与模型组GAS、MTL的含量分别为(28.61±0.12),(404.16±18.48)ng·L~(-1)比较,各药物组大鼠血清中GAS、MTL的含量分别为(34.14±1.47),(475.82±7.80);(35.12±3.40),(436.25±18.24);(34.77±2.65),(475.04±28.79);(33.28±3.58),(447.81±8.26);(35.26±4.89),(473.09±28.64);(36.02±5.12),(440.52±23.55);(35.20±4.55),(478.58±31.24);(34.88±5.07),(438.08±25.43)ng·L~(-1),差异均有统计学意义(均P<0.05)。结论甘肃不同产地黄芩根、茎叶水煎液均有一定的清热燥湿作用。
Objective To study the effects of heat and dampness and dampness on the root, stem and leaf of Scutellaria baicalensis in different habitats in Gansu province. Methods According to body weight, SD rats were randomly divided into blank group, model group, Radix Scutellariae group Ⅰ (control group), Longxi Scutellariae group Ⅱ (stem and leaf), Weiyuan baicalin Ⅰ group (Stems and leaves) in Zhang County, Radix Scutellariae group (root) in Zhang County, Radix Scutellariae group (stems and leaves) in Zhangxian County, Radix Scutellariae Group Ⅰ (Minus) in Min County, Radix Scutellariae Group Ⅱ group. Except for the blank group, the rats in other groups were given Feigan feeding and 56 degrees Red Star Erguotou gavage (5 mL · kg ~ (-1)) once a day and placed in an artificial climate chamber for 21 days On the 22nd day, E. coli with a concentration of 1 × 10 ~ 9 / mL was inoculated once (10μL · g ~ (-1)) for 4 days and once inactivated (5μL · g ~ (-1) , The establishment of rat damp-heat syndrome model. The rats in the blank group were given 0.9% NaCl 10 mL · kg -1. The rats in each drug group were given 10 g · kg -1 of Scutellariae Radix, Gavage, were 1 day, for 7 days. Syndromes of rats in each group were observed. Serum levels of interleukin-4 (IL-4), interferon gamma (IFN-γ), motilin (MTL) and gastrin (GAS) were measured by enzyme-linked immunosorbent assay Content and gastrointestinal tissue MTL, GAS content. Results Compared with the model group, the levels of IL-4 and IFN-γ were (38.09 ± 1.41) and (1203.90 ± 32.24) ng · L-1, respectively. The contents of IL-4 and IFN- Were (25.36 ± 2.56), (703.53 ± 29.36), (31.54 ± 3.81), (810.90 ± 24.07), (26.88 ± 2.91), (679.02 ± 30.72), (30.81 ± 3.19), (802.44 ± 20.69) (26.61 ± 3.94), (702.90 ± 14.86), (32.45 ± 5.12), (815.36 ± 34.78), (27.06 ± 4.01), (685.66 ± 42.05), (31.76 ± 3.98), (808.95 ± 38.22) ng · L ~ (-1), the differences were statistically significant (all P <0.05). Compared with model group, the content of GAS and MTL were (28.61 ± 0.12) and (404.16 ± 18.48) ng · L -1, respectively. The contents of GAS and MTL in each group were (34.14 ± 1.47) , (4.75.82 ± 7.80); (35.12 ± 3.40), (436.25 ± 18.24); (34.77 ± 2.65), (475.04 ± 28.79); (33.28 ± 3.58), (447.81 ± 8.26) 473.09 ± 28.64); (36.02 ± 5.12), (440.52 ± 23.55); (35.20 ± 4.55), (478.58 ± 31.24); (34.88 ± 5.07), (438.08 ± 25.43) ng · L -1, All were statistically significant (P <0.05). Conclusion Scutellariae from different areas of Gansu root, stem and leaf decoction have certain heat dampness effect.