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一般脑标本,多用瓶装液体保存,有时往往由于瓶口封闭不严,瓶内液体蒸发而降低质量。此外,液体保存的标本,使用时也不方便。因此,不少作者先后介绍了各种制作干标本的方法,企图弥补液体保存的缺陷。例如:Rack(1951)曾介绍过石蜡浸渍法;(1954)、横地千仞和大手裕(1957)等分别介绍过冰冻法;Kubik(1957)曾介绍过先用食盐福尔马林液固定,然后再用甘油浸泡的方法;Sakla(1959)还介绍了先用逐增浓度的福尔马林液固定,继之用酒精、丙酮、甘油混合液涂浸标本,然后晾干的方法。上述各种方法,各有利弊,如用石蜡浸渍法作出的标本,能够保持其体积不发生大的变化,但往往由于蜡液处理而使脑表面的结构不太明显;冰冻法制作的干标本往往体积收缩较剧。Kubik和Sakla介绍的方法比前两种好,做出的标本既能使体积变化不大,又能使表面结构清晰,但是,需要较长的时间才能得到令人满意的结果。因此,我们试用了热甘油浸渍法制作脑的干标
General brain specimens, multi-purpose bottle of liquid preservation, sometimes due to the bottleneck is not closed, the bottle of liquid evaporation and reduce the quality. In addition, the liquid preserved specimens, inconvenient to use. Therefore, many authors have introduced a variety of methods for making dry specimens in an attempt to make up for the shortcomings of liquid preservation. For example, Rack (1951) introduced paraffin wax impregnation; (1954), Hirokazu Hiroshi and Ohama Hiroyuki (1957) introduced the method of freezing; and Kubik (1957) And then soaked with glycerol; Sakla (1959) also introduced the first by increasing the concentration of formalin fixation, followed by alcohol, acetone, glycerol mixture coated specimens, and then dried. The various methods mentioned above have their own advantages and disadvantages, such as specimens made by paraffin impregnation method, which can keep their volume unchanged. However, the structure of the brain surface is often not obvious due to the wax solution treatment. Volume contraction is often more drama. The methods described by Kubik and Sakla are better than those of the first two, and the specimens made are not only small in volume but also clear in surface structure, but take longer to obtain satisfactory results. Therefore, we experimented with the hot glycerol impregnation method for making brain stems