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作者应用鼠伤寒沙门氏菌回变试验、小鼠骨髓细胞微核试验及CHO细胞染色畸变试验,对国产氨利酮与甲腈吡酮进行诱变性试验研究。结果:氨利酮和甲腈吡酮在鼠伤寒沙门氏菌回变试验均为阴性,CHO细胞染色体畸变试验均为阳性;氨利酮剂量为其临床有效剂量的70～210倍时,小鼠骨髓细胞微核试验为阳性,甲腈吡酮则为阴性。提示该两药均不引起基因突变,但氨利酮可能在体内、外引起染色体损伤,甲腈吡酮在体外引起染色体损伤,可能通过体内代谢变弱,故在诱变性方面甲腈吡酮可能比氨利酮更为安全。
The author applied mutagenicity test of Salmonella typhimurium, micronucleus test of mouse bone marrow cells and staining of CHO cells to make the mutagenicity test of domestic ammonia ketone and tetramethylpyrolidone. Results: Both of aminarin and tetracimide were negative in the mutagenicity test of Salmonella typhimurium, and the chromosomal aberration test of CHO cells was positive. When the dose of ammonia was 70 ~ 210 times of the clinical effective dose, the mouse bone marrow cells Micronucleus test was positive, while aconitine was negative. Suggesting that the two drugs do not cause mutations, but ammonia ketone may cause chromosomal damage in vitro and in vivo, tetracycline induced chromosomal damage in vitro, may be weakened metabolism in the body, so the mutagenic aspects of acteoside May be safer than aminone.