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目的：研究一叶秋碱能否诱导ＨＬ６０细胞凋亡．方法：用ＭＴＴ法检测一叶秋碱对细胞增殖影响；应用流式细胞仪检测凋亡细胞数；采用琼脂糖凝胶电泳法观测ＤＮＡ碎片；透射电镜观察凋亡的形态改变．结果：一叶秋碱５－８０ｍｇ·Ｌ－１能诱导ＨＬ６０细胞凋亡．电镜观察到典型的凋亡形态学改变，电泳呈现出阶梯状条带，流式细胞仪检测到凋亡率随剂量的增高而升高．ＭＴＴ法示一叶秋碱抑制ＨＬ６０细胞增殖，并且呈时间、剂量依赖性，药物作用１２ｈ的ＩＣ５０（９５％可信区间）分别为２７（１５－４７）ｍｇ·Ｌ－１．结论：一叶秋碱诱导ＨＬ６０细胞凋亡
Aim: To study whether Akaline could induce HL60 cell apoptosis. Methods: MTT assay was used to determine the effect of proctamine on cell proliferation. Flow cytometry was used to detect the number of apoptotic cells. DNA fragmentation was observed by agarose gel electrophoresis. Morphological changes of apoptotic cells were observed by transmission electron microscopy. Results: Akebia alkali 5-80mg · L-1 can induce HL 60 cell apoptosis. The morphological changes of typical apoptotic cells were observed under electron microscope. The electrophoresis showed a ladder-shaped band. The apoptotic rate was increased with the increase of the dosage by flow cytometry. MTT method showed a leaf fall alkali inhibited the proliferation of HL 60 cells in a time-and dose-dependent manner. The IC50 (95% confidence interval) of the drug for 12 hours was 27 (15-47) mg · L -1, respectively. Conclusion: Alokide induced HL 60 cell apoptosis