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一次成苗法培养效果与2.4—D浓度密切相关:在0.025～O.1mg/L范围内与2 mg/L多级比较,以0.025mg/L最高。籼粳杂种为材料,以花药为单位的出苗率为1.47％,绿苗率0.61％,比多级培养增0.06％与0.03％,以培养试管为单位的出苗率为69.05％,绿苗率为28.57％,比多级培养提高55.09％与20.88％。培养效果试验,同样培养管数,出苗率比多级法提高1.68倍与1.05倍。雄核发育成苗,同时存在愈伤组织再分化与形成胚状体二种途径。
The result of one-time seedling culture was closely related to the concentration of 2.4-D: 0.025 ~ 0.1 mg / L, compared with 2 mg / L multistage, the highest was 0.025 mg / L. Indica-japonica hybrids as materials, the anther emergence rate was 1.47%, the green seedling rate was 0.61%, which was 0.06% and 0.03% higher than that of the multi-level culture. The germination rate of the test tube was 69.05% 28.57%, which is 55.09% and 20.88% higher than the multi-level culture. Cultivation effect test, the same culture tube number, the emergence rate than the multi-level method to improve 1.68 times and 1.05 times. Male nucleus develop into seedlings, there are two ways of callus differentiation and formation of embryoid body.