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目的通过观察大鼠阴茎海绵体组织中晚期糖基化终产物(AGEs)及其受体(RAGE)的变化对氧自由基代谢的影响,探讨AGEs在糖尿病性勃起功能障碍(DMED)发生发展中的作用。方法成年雄性SD大鼠60只,随机取40只用于制作糖尿病模型,造模成功的大鼠分为两组:糖尿病(DM)组和糖尿病+氨基胍给药(DM+AG)组;另20只大鼠亦分为两组:正常对照(CO)组和正常对照+氨基胍给药(CO+AG)组;氨基胍(AG)给药组大鼠造模后即在其饮水中按1g/L剂量加入AG。饲养8周后取各组大鼠阴茎海绵体组织,一小段用于免疫组化观察分析AGEs及其受体的分布和表达,剩余部分匀浆后检测AGE-肽(AGE-P)含量、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果DM组阴茎海绵体组织中AGEs和RAGE的表达、AGE-P含量及MDA含量明显高于CO组(P<0.05),而SOD活性则明显低于后者(P<0.05),而AG则明显减少了DM大鼠阴茎海绵体组织中AGEs和RAGE表达、AGE-P及MDA的生成,增强了SOD活性;CO组与CO+AG组间比较在各项指标上则无明显差异(P>0.05)。结论糖尿病状态下AGEs与RAGE的结合效应可以引起大鼠阴茎海绵体组织中氧自由基的产生增多,抗氧化能力的下降,从而促进DMED的发生发展。
OBJECTIVE: To investigate the effects of AGEs and its receptor (RAGE) on the metabolism of oxygen free radicals in rats with corpus cavernosum and to explore the role of AGEs in the development of diabetic erectile dysfunction (DMED) Role. Methods Sixty adult male Sprague-Dawley rats were randomly divided into four groups. The rats were divided into two groups: DM group and DM + AG group. Twenty rats were also divided into two groups: normal control (CO) group and normal control + aminoguanidine administration (CO + AG) group; aminoguanidine (AG) administration group rats were 1g / L dose added to AG. After feeding for 8 weeks, the corpus cavernosum tissues of each group were taken, and a small section was used to observe the distribution and expression of AGEs and its receptors by immunohistochemical staining. The remaining part of the cells were tested for AGE-P (AGE-P) Dialdehyde (MDA) content and superoxide dismutase (SOD) activity. Results The expressions of AGEs and RAGE, the content of AGE-P and the content of MDA in DM group were significantly higher than those in CO group (P <0.05), while the activities of SOD in DM group were significantly lower than those in the latter group (P <0.05) Significantly reduced the expression of AGEs and RAGE, AGE-P and MDA in the corpus cavernosum of DM rats, and enhanced the activity of SOD. There was no significant difference between CO and CO + AG groups (P> 0.05). Conclusions The combined effect of AGEs and RAGE in diabetic condition can induce the increase of oxygen free radicals and the decrease of antioxidative capacity in the corpus cavernosum, thus promoting the development of DMED.