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通过拉曼光谱技术测定了液体小檗碱(Berberine)的常规拉曼光谱(NRS)及表面增强拉曼光谱(SERS),归属了拉曼特征峰,并与固体小檗碱的NRS进行对比,发现特征峰位基本没变.研究小檗碱与纳米银(Ag-Berberine)、包裹牛血清白蛋白(BSA)的线状银(Ag@BSA-Berberine)的拉曼光谱,分析了线性纳米银(Ag)及包裹BSA的线状银(Ag@BSA)对小檗碱的吸附模型.结果显示,线性纳米银对小檗碱、牛血清白蛋白有明显的拉曼增强效应,增强效应主要在线性纳米银与氨基酸中N的孤电子对、甲氧基中的氧、苯环中的π电子及异喹啉中的N发生了吸附作用.对比Ag@BSA-Berberine和Ag-Berberine的特征峰,加了BSA后的拉曼光谱有明显的减弱,但峰位并没有移动,但是有BSA修饰的纳米银的小檗碱拉曼光谱有较高的稳定性,利于小檗碱的进一步研究.
Raman spectroscopy was used to determine the regular Raman spectra (NRS) and surface enhanced Raman spectroscopy (SERS) of the liquid berberine. The Raman peaks were assigned and compared with the NRS of the solid berberine. And found that the characteristic peak has not changed.We studied the Raman spectra of berberine and Ag-Berberine and BSA-coated linear silver (Ag @ BSA-Berberine) (Ag) and linear silver loaded BSA (Ag @ BSA) on berberine.The results show that the linear nano-silver on berberine, bovine serum albumin Raman enhancement significantly enhanced effect of the main line Ag nanosilver was adsorbed by N in the amino acid, oxygen in the methoxy group, π electron in the benzene ring and N in isoquinoline.Comparing the characteristic peaks of Ag @ BSA-Berberine and Ag-Berberine The Raman spectrum of BSA was obviously weakened, but the peak position did not move. However, the Raman spectra of berberine with BSA modified nano silver showed higher stability, which was benefit to the further study of berberine.