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目的 分析大肠癌组织中Survivin、MSH6、MSH2表达变化,探讨其与临床病理参数之间的关系.方法 采用免疫组化法检测汉中市中心医院的197例大肠腺癌标本及20例炎性肠黏膜中Survivin、MSH6、MSH2的蛋白表达,分析其与大肠癌临床病理特征的关系及三者表达的相关性 20例炎性非肿瘤肠黏膜组织(对照组)中,MSH2阳性表达率为95％,MSH6阳性表达率为95％,Survivin阳性表达率为10％;197例大肠癌组中MSH2阳性表达率88.3％,MSH6阳性表达率74.1％,Survivin阳性表达率84.3％;大肠癌组织中Survivin阳性表达率明显高于炎性对照组(P＜0.05).大肠癌组织中Survivin表达与浸润深度和淋巴结转移有关(P＜0.05),与性别、年龄、肿瘤大小、肉眼类型及分化程度无关(P＞0.05);MSH2表达与肿瘤大小和淋巴结转移有关(P＜0.05),与性别、年龄、肉眼类型、浸润深度及分化程度无关(P＞0.05);MSH6表达与性别和淋巴结转移有关(P＜0.05),与年龄、肿瘤大小、肉眼类型、浸润深度及分化程度无关(P＞0.05).大肠癌组织中MSH6与MSH2表达存在正相关(r=0.326,P＜0.01),MSH2与Survivin阳性表达存在正相关(r=0.277,P＜0.01),MSH6与Survivin阳性表达存在正相关(r=0.435,P＜0.01).结论 大肠癌组织中Survivin 呈现高表达,临床上对MSH2、MSH6、Survivin检测有助于大肠癌转移风险、预后评估、及临床治疗提供依据,特别是Survivin基因可能为基因治疗提供依据.“,”Objective To analyze the changes of Survivin,MSH6 and MSH2 expression in colorectal cancer and explore their relationship with clinical and pathological parameters.Methods 197 cases of colorectal adenocarcinoma and 20 cases of inflammatory intestinal mucosa were detected by immunohistochemistry with Survivin,MSH6 and MSH2,and the correlation between Survivin,MSH6 and MSH2 expression was analyzed.Results In the control group of 20 cases with inflammatory non-tumor intestinal mucosa,the positive expression rate of MSH2,MSH6,Survivin were 95％,95％,10％,respectively.While the positive expression rate of MSH2,MSH6,Survivin were 88.3％,74.1％ and 84.3％ in 197 cases of colorectal cancer.Survivin positive expression rate in colorectal cancer group was significantly higher than that in inflammatory control group (P ＜ 0.05).Survivin expression was correlated with invasion depth and lymph node metastasis in colorectal cancer tissue (P ＜ 0.05),but not with gender,age,tumor size,gross type or degree of differentiation (P ＞ 0.05).MSH2 expression was correlated with tumor size and lymph node metastasis (P ＜ 0.05),but not with gender,age,gross type,depth of infiltration and degree of differentiation (P ＞ 0.05).MSH6 expression was related to gender and lymph node metastasis (P ＜ 0.05),but not to age,tumor size,gross type,infiltration depth and differentiation (P ＞ 0.05).Colorectal cancer tissues showed positive correlation between MSH6 and MSH2 (r =0.326,P ＜ 0.01),positive correlation between MSH2 and Survivin positive expression (r =0.277,P ＜ 0.01),and positive correlation between MSH6 and Survivin positive expression (r =0.435,P ＜ 0.01).Conclusions The positive expression rate of Survivin in colorectal cancer is high.MSH2,MSH6 and Survivin in colorectal cancer play an important role in the development and progression of colorectal cancer,and can provide evidence for the detection of these three factors,including metastasis risk,prognosis assessment and clinical treatment.In particular,Survivin gene may provide evidence for gene therapy.