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测定细菌对抗菌素敏感性试验是临床上选用抗菌素的重要依据。目前,测定的方法是试管稀释法、琼脂内扩散法和琼脂内稀释法,三种方法各有其优缺点。我们认为干燥滤纸片琼脂内扩散法较为实用。干燥滤纸片法的单种抗菌素敏感性试验及联合抗菌素敏感性试验在国内已有报告。我们参考一些文献,设计了联合抗菌素敏感性试验的干燥滤纸片法。并测定50株细菌的敏感性,同时与试管稀释法对照,效果尚满意,现将初步实验结果报告如下。
Determination of bacteria on antibiotic susceptibility testing is an important basis for clinical use of antibiotics. At present, the test method is the tube dilution method, agar diffusion method and agar dilution method, the three methods have their advantages and disadvantages. We think dry filter paper agar diffusion method is more practical. Dry filter paper single antibiotic susceptibility testing and combined antibiotic susceptibility tests have been reported in China. With reference to some literature, we designed a dry filter paper method that combines antibiotic susceptibility testing. And the sensitivity of 50 strains of bacteria was determined. At the same time, it was compared with the test tube dilution method, and the effect was still satisfactory. The preliminary experimental results are reported as follows.