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以 4个姬松茸菌株和 2个双孢蘑菇菌株为材料 ,用 2 0个随机引物对它们进行RAPD扩增。通过对供试菌株遗传相似系数的估算和系统聚类分析 ,构建姬松茸和双孢蘑菇遗传相关树状聚类图谱。结果表明 ,进行RAPD扩增的 2 0个随机引物中 ,有 12个能产生三种带型。当遗传相似系数升至 0 .8713时 ,这 6个菌株被分为 3类 ,第一类为菌株 18和 2 6 ,第二类为菌株 13和 12 ,第三类为菌株 33和 4 5。
Four Agaricus blazei strains and two Agaricus bisporus strains were used as materials, and 20 random primers were used to amplify them by RAPD. Based on the genetic similarity coefficient estimation and phylogenetic analysis of the tested strains, the genetic correlation dendrogram of Agaricus blazei and Agaricus bisporus was constructed. The results showed that 12 out of 20 random primers for RAPD amplification could produce three bands. When the genetic similarity coefficient rose to 0.8713, the six strains were divided into three groups, the first being strains 18 and 26, the second being strains 13 and 12, and the third being strains 33 and 45.