论文部分内容阅读
目的探讨三斑海马液化蛋白液的抗氧化活性。方法酶法提取工艺采用了4组单因素实验、16组正交试验进行优化,以对DPPH自由基的清除率来衡量三斑海马液化蛋白的抗氧化活性。结果三斑海马在料液比1:10、反应时间6h、酶用量1%时液化蛋白水解率最高,提取物对DPPH自由基的抗氧化活性最强。结论初步探明三斑海马液化蛋白的提取方法及水解液对DPPH的抗氧化活性,为开发海马提供理论基础。
Objective To investigate the antioxidant activity of three-spot hippocampal liquefied protein solution. Methods Enzymatic extraction was carried out using four groups of single factor experiments and 16 orthogonal experiments. The DPPH radical scavenging rate was used to measure the antioxidant activity of the three-spot hippocampal liquefaction protein. Results The hippocampus had the highest liquefaction rate when the ratio of material to liquid was 1:10, the reaction time was 6h and the amount of enzyme was 1%. The extract had the strongest antioxidant activity on DPPH free radical. Conclusion The method of extracting liquefied protein from three-spot hippocampus and the antioxidant activity of hydrolyzate against DPPH were preliminary studied, providing a theoretical basis for the development of hippocampus.