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目的观察消癥通络中药对糖尿病肾病大鼠胃排空的干预作用并探讨其可能机制。方法选择雄性SD大鼠50只,随机分为空白组、模型组、中药低剂量组、中药中剂量组、中药高剂量组,用左肾切除术加腹腔注射链脲佐菌素制作糖尿病肾病模型,中药低、中、高剂量组分别灌服消癥通络中药5.29g/(kg.d)、10.58g/(kg.d)、21.16g/(kg.d),空白组、模型组大鼠灌服相当剂量的生理盐水,连续6周。实验结束测定胃排空率、血清瘦素、胰高血糖素水平。结果与空白组比较,模型组大鼠胃排空率明显下降(P<0.05),血清瘦素、胰高血糖素水平明显升高;与模型组比较,消癥通络中药能明显改善糖尿病大鼠胃排空,明显降低大鼠血清瘦素、胰高血糖素水平(P<0.05);血清瘦素、胰高血糖素水平与胃排空率呈明显负相关。结论消癥通络中药能明显改善糖尿病肾病大鼠胃排空功能,其机制可能与降低大鼠血清瘦素、胰高血糖素水平有关。
Objective To observe the intervention of Xiaotong Tongluo Chinese medicine on gastric emptying in diabetic nephropathy rats and to explore its possible mechanism. Methods Fifty male Sprague-Dawley rats were randomly divided into blank group, model group, low-dose Chinese medicine group, medium-dose Chinese medicine group and high-dose Chinese medicine group. Diabetic nephropathy model was induced by left nephrectomy plus intraperitoneal injection of streptozotocin , Low dose, middle dose and high dose of traditional Chinese medicine group were given Xiazhong Tongluo Chinese traditional medicine 5.29g / (kg · d), 10.58g / (kg · d), 21.16g / (kg · d) Rats fed a dose of saline for 6 weeks. Gastric emptying rate, serum leptin and glucagon were measured at the end of the experiment. Results Compared with the blank group, the gastric emptying rate of the model group decreased significantly (P <0.05) and the level of serum leptin and glucagon increased significantly. Compared with the model group, Xiaoxuetongluo Chinese medicine can significantly improve the diabetes mellitus Rats gastric emptying significantly reduced serum leptin and glucagon levels (P <0.05); serum leptin and glucagon levels were significantly negatively correlated with gastric emptying rate. Conclusion Xiaoxuetongluo can significantly improve gastric emptying in diabetic nephropathy rats, which may be related to the reduction of serum leptin and glucagon in rats.