论文部分内容阅读
目的通过分析分离自病毒性脑炎病人的一株柯萨奇病毒B3株KMA103-09生物学特性,研究其致病机理。方法将KMA103-09病毒分别按一定量接种至KMB17细胞、RD细胞、VERO细胞、Hep-2细胞、Hela细胞及MRC-5细胞进行培养,观察细胞的生长情况,并采用微滴法检测病毒在不同时间、不同细胞中的病毒感染滴度;将一定量KMA103-09病毒注射乳鼠和成鼠脑内,观察病毒的致病性,并检查鼠血清中IL-2、IL-4、IL-6、IFN-r、TNF、IL-17A、IL-10含量。结果该病毒株在6种细胞上皆能适应生长,并产生明显的细胞病变效应。病毒在Hela细胞上生长繁殖最快,42h时细胞全部发生病变,感染性滴度达8.625lgCCID50/ml;在MRC-5细胞上病毒的感染性滴度最低,120h才全部发生病变,感染性滴度为5.50lgCCID50/ml。该病毒对一日龄乳鼠致死并在脑、肺和心脏等组织产生病理改变,实验乳鼠血清IL-6、IL-10、TNF含量分别为4.71pg/ml、21.08pg/ml和36.79pg/ml,与阴性对照组比较差异有统计学意义(P<0.05),其余4种皆无表达。该病毒对成鼠不致病,脑、肺、心脏病理检查无异常。结论 KMA103-09病毒在Hela细胞生长适应性良好,对乳鼠致病而对成鼠不致病。
OBJECTIVE: To study the pathogenic mechanism of KMA103-09, a strain of coxsackievirus B3 isolated from patients with viral encephalitis. Methods KMA103-09 were inoculated into KMB17 cells, RD cells, VERO cells, Hep-2 cells, Hela cells and MRC-5 cells respectively. The growth of the cells was observed. Different time, different cells in the virus infection titers; a certain amount of KMA103-09 virus injected into the rat and rat brain, observed the virus pathogenicity, and check the serum of rats IL-2, IL-4, IL- 6, IFN-r, TNF, IL-17A, IL-10 content. The results of the virus strains in all six kinds of cells can adapt to growth and produce significant cytopathic effect. The virus grew and breed fastest on Hela cells. The cells all developed lesions at 42h. The infectious titer was 8.625lgCCID50 / ml. The infectious titer of MRV-5 cells was the lowest at 120h, and all the lesions were infected. Degree of 5.50lgCCID50 / ml. The virus caused the death of one-day-old suckling mice and had pathological changes in brain, lung and heart tissues. The contents of IL-6, IL-10 and TNF in experimental suckling mice were 4.71pg / ml, 21.08pg / ml and 36.79pg / ml, compared with the negative control group, the difference was statistically significant (P <0.05), the remaining 4 were no expression. The virus is not pathogenic to rats, brain, lung, heart pathology check no abnormalities. Conclusion The KMA103-09 virus has good adaptability to Hela cell growth, pathogenic to neonatal mice and non-pathogenic to mice.