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做法是:在接种箱内的酒精灯旁,放置一长形小瓶,内装75％酒精。经酒精灯火焰烧红的接种铲插入75％酒精瓶中,稍停数秒接种铲很快冷却。取出接种铲通过火焰,点燃后立即移开,沾附在接种铲上的酒精燃烧后,自行熄灭。此时,接种铲经火焰灭菌,又经75％酒精灭菌,再点燃沾附在接种铲上的酒精三次灭菌,完全达到无菌效果,而且接种铲不热。经此法灭菌的接种铲可连续移接数次,再如法灭菌。此法既保证接种铲灭
The practice is: next to the alcohol lamp in the inoculation box, place an elongated vial containing 75% alcohol. After the alcohol lamp flame burning red vaccination shovel inserted into 75% alcohol bottle, stop the vaccination shovel a few seconds to cool down quickly. Remove the vaccination shovel through the flame, remove immediately after ignition, the alcohol attached to the vaccination shovel, go out on their own. At this point, the vaccination shovel is sterilized by flame, sterilized with 75% alcohol, and then sterilized three times with alcohol attached to the inoculated spatula to achieve a complete germ-free effect. The vaccination shovel is not hot. Inoculated shovels sterilized by this method can be continuously transferred several times, and then sterilized. This method is not only guaranteed vaccination shovel out