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芒果细菌性黑斑病是由野油菜黄单胞菌芒果致病变种(Xanthomonas campestris pv.mangiferaeindicae)引起的细菌性病害,是芒果生产中最为重要的病害之一。本试验通过对比已报道的黄单胞属的抗铜基因copA序列的分析、比较,设计简并引物Copa-F/Copa-R,采用同源克隆法从芒果细菌性黑斑病病菌中扩增出约1 800 bp的DNA片段。序列分析表明,该基因片段含有1个完整的copA同源基因(GenBank accession No.KJ890405.1),其核苷酸序列全长1 794 bp,编码597个氨基酸,其分子量约66.271 kDa,等电点(PI)为6.40。系统进化树结果显示,该基因编码的氨基酸序列与Xanthomonas属多个不同种的抗铜基因聚为一类。
Bacterial black spot disease of mango is a bacterial disease caused by Xanthomonas campestris pv. Mangiferaeindicae and is one of the most important diseases in mango production. In this study, Copa-F / Copa-R, a degenerate primer, was designed and synthesized by comparing the reported copA sequences of the resistance genes of the genus Xanthomonas. The homologous cloning method was used to amplify the CopA- Approximately 1 800 bp of DNA fragments were produced. Sequence analysis showed that the gene fragment contained a complete copA homologous gene (GenBank accession No. KJ890405.1), which contained a full-length 1 794 bp nucleotide sequence encoding 597 amino acids with a molecular weight of about 66.271 kDa and an isoelectric point The point (PI) is 6.40. Phylogenetic tree analysis showed that the amino acid sequence encoded by this gene was clustered with a number of different copper resistance genes belonging to the genus Xanthomonas.