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Purpose: The present study aimed to investigate the reliability of the non-exhaustive double effort (NEDE) test in running exercise and its associations with the ventilatory thresholds(VT1and VT2)and the maximal lactate steady state(MLSS). Methods:Ten healthy male adults(age:23 ± 4 years,height:176.6 ± 6.4 cm,body mass:76.6 ± 10.7 kg)performed 4 procedures:(1)a ramp test for VT1and VT2determinations measured by ratio of expired ventilation to O2uptake (VE/VO2) and expired ventilation to CO2output (VE/VCO2) equivalents, respectively; (2) the NEDE test measured by blood lactate concentration (NEDELAC) and heart rate responses (NEDEHR);(3)a retest of NEDE for reliability analysis;and(4)continuous efforts to determine the MLSS intensity.The NEDE test consisted of 4 sessions at different running intensities.Each session was characterized by double efforts at the same running velocity(E1 and E2,180 s),sepa-rated by a passive recovery period(90 s rest).LAC and HR values after E1 and E2(in 4 sessions)were used to estimate the intensity equivalent tonull deltaby linear fit.This parameter represents,theoretically,the intensity equivalent to maximal aerobic capacity. Results:The intraclass correlation coefficient indicated significant reliability for NEDELAC(0.93)and NEDEHR(0.79)(both p<0.05).There were significant correlations,no differences,and strong agreement with the intensities predicted by NEDELAC(10.1 ± 1.9 km/h)and NEDEHR (9.8 ± 2.0 km/h)to VT1(10.2 ± 1.1 km/h).In addition,despite significantly lower MLSS intensity(12.2 ± 1.2 km/h),NEDELACand NEDEHR intensities were highly correlated with this parameter(0.90 and 0.88,respectively). Conclusion:The NEDE test applied to running exercise is reliable and estimates the VT1intensity.Additionally,NEDE intensities were lower but still correlated with VT2and MLSS.