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The in vitro oxidative hemolysis of human red blood cells (RBC) was used as a model to study the free radical induced damage of biological membranes and the inhibitory effect of natural antioxidants. The hemolysis was induced by a water-soluble free radical initiator 2,2’-azo(2-asmidinopropane)dihydrochloride (AAPH) and inhibited by the principal polyphenolic components extracted from green tea leaves, i.e. (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicat-echin gallate (ECG), (-)-epigallocatechin gallate (EGCG) and gallic acid (GA). Addition of AAPH at 37℃ caused fast hemolysis after a short period of inhibition period, while addition of the green tea polyphenols efficiently suppressed the hemolysis in the activity sequence of EGCG > EGC> ECG ≈EC>GA, demonstrating that these green tea polyphenols are effective antioxidants which could protect biological membranes from free radical induced oxidative damage.
The in vitro oxidative hemolysis of human red blood cells (RBC) was used as a model to study the free radical induced damage of biological cells and the inhibitory effect of natural antioxidants. The hemolysis was induced by a water-soluble free radical initiator 2, (-) - epigallocatechin (EGC), (-) - epicat (2-asmidinopropane) dihydrochloride (AAPH) and inhibited by the principal polyphenolic components extracted from green tea leaves, ie -echin gallate (ECG), (-) - epigallocatechin gallate (EGCG) and gallic acid (GA). Addition of AAPH at 37 ° C caused rapid hemolysis after a short period of inhibition period, while addition of the green tea polyphenols efficiently suppressed the hemolysis in the activity sequence of EGCG> EGC> ECG ≈ EC> GA, demonstrating that these green tea polyphenols are effective antioxidants which could protect biological membranes from free radical induced oxidative damage.