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目的:研究血必净在OVA诱导的小鼠变应性鼻炎(AR)模型中的干预作用及其对血红素加氧酶-1(HO-1)表达的影响,探讨血必净注射液气道局部雾化吸入给药的可行性。方法:建立OVA诱导的小鼠AR模型,用血必净[1ml/(kg·d)]和地塞米松[30mg/(kg·d)]分别进行气道雾化治疗,对照组用PBS(pH 7.4)代替;检测各组小鼠鼻腔灌洗液中嗜酸粒细胞计数;ELISA法检测鼻腔灌洗液中炎性因子、OVA特异性IgE的表达水平;苏木精-伊红染色法检测鼻黏膜及肺组织的形态学变化;用Western Blot、Real-time PCR及免疫组织化学检测鼻黏膜及肺组织中HO-1的表达。结果:经血必净气道局部雾化吸入给药处理的小鼠可明显降低气道中的嗜酸粒细胞、炎性因子IL-4、IL-5、IL-13和TNF-α的表达水平,提高IFN-γ的表达水平;降低鼻腔灌洗液中OVA特异性IgE的表达水平;减少鼻黏膜和肺组织中黏液产生和炎症细胞的浸润;诱导鼻黏膜及肺组织HO-1的表达。结论:血必净注射液气道局部雾化吸入给药可以抑制OVA诱导的小鼠AR模型中炎性因子产生,逆转Th1/Th2失衡,其治疗作用可能与诱导鼻黏膜组织HO-1的高表达有关。
Objective: To investigate the effect of Xuebijing in the OVA-induced mouse model of allergic rhinitis (AR) and its effect on the expression of heme oxygenase-1 (HO-1) Local aerosol inhalation administration feasibility. Methods: The OVA-induced AR model was established in mice. Airway atomization was performed with Xuebijing [1ml / (kg · d)] and dexamethasone [30mg / (kg · d) pH 7.4). The numbers of eosinophils in nasal lavage fluid of mice in each group were measured. The levels of inflammatory cytokines and OVA-specific IgE in nasal lavage fluid were detected by ELISA. Nasal mucosa and lung tissue. The expression of HO-1 in nasal mucosa and lung tissue was detected by Western Blot, Real-time PCR and immunohistochemistry. Results: Mice treated with local nebulized inhalation of meridian-induced net airway could significantly reduce the levels of eosinophils, IL-4, IL-5, IL-13 and TNF- Increase the level of IFN-γ, reduce the expression level of OVA-specific IgE in nasal lavage fluid, decrease the mucus production and inflammatory cell infiltration in nasal mucosa and lung tissue, and induce the expression of HO-1 in nasal mucosa and lung tissue. Conclusion: Local aerosol inhalation of Xuebijing injection can inhibit the production of inflammatory cytokines in OVA-induced mouse AR model and reverse the imbalance of Th1 / Th2, which may be related to the induction of HO-1 in nasal mucosa Expression related.