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目的观察~(125)Ⅰ粒子近距离照射对H22肝癌细胞凋亡的影响及其机制。方法用原位末端脱氧核苷酸转移酶标记法(TUNEL)检测~(125)Ⅰ粒子近距离照射对H22细胞凋亡的影响;免疫组化Elivion ~(TM)plus法检测Survivin蛋白的表达。60只小鼠分为A组:植入放射性粒子;B组:植入化疗药DDP;C组:植入放射性粒子和化疗药DDP;D组:正常对照组。结果~(125)Ⅰ粒子近距离照射和/或化疗后对H22肝癌肿瘤体积抑制率A、B、C组分别为43.8%、40.7%和58.3%;凋亡指数(AI)分别为(25.15±10.36)、(33.42±12.25)和(42.34±13.95),与对照组D组(20.45±14.54)比较,C组其凋亡指数(AI)差异有统计学意义(P<0.05);Survivin蛋白的表达率分别为50.0%、55.6%和36.4%,与D组100.0%比较,C组表达率差异有统计学意义(P<0.05)。结论~(125)Ⅰ粒子近距离照射H22肝癌可有效诱导肝癌细胞凋亡,抑制肝癌细胞增殖;联合化疗药,其凋亡作用更强;Sur- vivin可能参与了其中的调节作用。
Objective To observe the effect of ~ (125) Ⅰ particles on the apoptosis of H22 hepatocarcinoma cells and its mechanism. Methods The apoptosis of H22 cells was detected by TUNEL method. The expression of Survivin protein was detected by immunohistochemical Elivion ™ (TM) plus method. 60 mice were divided into group A: radioactive particles implantation; group B: implantation of chemotherapy drug DDP; group C: implantation of radioactive particles and chemotherapy drug DDP; group D: normal control group. Results The tumor volume inhibition rates of H22 hepatocellular carcinoma were 43.8%, 40.7% and 58.3% respectively after irradiation with ~ (125) Ⅰ particles and / or chemotherapy. The apoptotic index (AI) 10.36), (33.42 ± 12.25) and (42.34 ± 13.95), respectively. Compared with control group D (20.45 ± 14.54), the apoptotic index (AI) in group C was significantly different (P <0.05); Survivin protein The expression rates were 50.0%, 55.6% and 36.4% respectively. Compared with 100.0% in group D, the expression rates in group C were significantly different (P <0.05). CONCLUSION: The close irradiation of ~ (125) Ⅰ particles with H22 hepatocarcinoma can effectively induce the apoptosis of hepatocellular carcinoma cells and inhibit the proliferation of hepatocellular carcinoma cells. The combination of chemotherapeutic agents has stronger apoptosis effect. Survivin may be involved in the regulation.