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目的:探讨准噶尔乌头对生物体各器官可能造成的毒性及相应生化指标的变化规律,并比较药材不同提取部位之间毒性的异同。方法:分别对准噶尔乌头乙酸乙酯提取部位与乙醇部位进行全自动生化分析,采用SPSS 13.0统计软件分析各项生化指标的变化规律;再利用SIMCA14.0软件对生化指标开展多元变量分析,及PCA、PLS-DA和OPLS-DA模式识别,阐明生化指标随时间的区分度和差异性,及不同提取部位的标志性生化指标。结果:该药材对肝与肾的毒性较大,标志性生化指标均为LDH与AST,其中乙醇部位引起所有指标在2h内的变化与振荡更加剧烈,且在6h~12h之间又出现反复波动,对生物体的影响更加明显。结论:在临床上使用该类药材时应该重视对肝、肾的保护措施,或者是对肝与肾的恢复治疗;且当此类药材中毒时尽可能在2h内采取急救措施。
OBJECTIVE: To investigate the possible changes of the toxicity and corresponding biochemical indexes of various organs of Zunggar Aconitum, and to compare the similarities and differences of toxicity between different parts of Radix Astragali. Methods: Fully automatic biochemical analysis was performed on the parts of ethanol extract and ethyl alcohol extract from the germplasm of Fructus Junlichee. The changes of biochemical parameters were analyzed by SPSS 13.0 software. Then the multivariate analysis of biochemical parameters was carried out by SIMCA14.0 software. PCA, PLS-DA and OPLS-DA pattern recognition, clarify the distinction and difference of biochemical indicators over time, and the biochemical indicators of different extraction sites. Results: The drug was more toxic to liver and kidney, and the biochemical markers were all LDH and AST, in which the changes and oscillations of all the indicators caused by ethanol were more severe in 2h, and fluctuated repeatedly between 6h and 12h , The impact on the organism more obvious. Conclusion: When using this kind of medicinal materials clinically, we should pay more attention to the protective measures on the liver and kidneys or restore the liver and kidneys. And when the poisoning of these medicinal materials should be taken within 2 hours, first aid measures should be taken.