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目的:探讨血清中CREG蛋白在急性心肌梗死发作早期的表达情况,尝试为临床心肌缺血的极早期诊断提供一种新的血清标志分子。方法:在2010年6月至2010年11月期间,入选在沈阳军区总医院心内科住院治疗的急性ST段抬高型心肌梗死患者50例及非AMI对照50例,于AMI组胸痛发作后的不同时间点采血测定CK、CK-MB、LDH和cTnT,同时应用Western blot技术测定血清中CREG蛋白的含量,并与对照组比较。结果:AMI组发病72小时内的血清中CREG蛋白表达均较对照组有不同程度的增高(P<0.05)。胸痛开始2h内,AMI组血清中CREG的含量即明显增高,其在2h、4h及6h的含量显著高于对照组(P<0.001)。在胸痛已经发作2小时内,两组间血清cTnT、CK、CK-MB及LDH水平比较无统计学意义(P>0.05)。结论:CREG在AMI患者血清中的表达增高,其在血清中表达时间早于cTNT及CK-MB。
Objective: To investigate the expression of serum CREG protein in the early stage of acute myocardial infarction (AMI) and to provide a new serum marker for early diagnosis of clinical myocardial ischemia. Methods: From June 2010 to November 2010, 50 patients with acute ST-segment elevation myocardial infarction and 50 patients without AMI hospitalized in Department of Cardiology, Shenyang Military Region General Hospital were enrolled in this study. After AMI patients had chest pain Blood samples were collected for determination of CK, CK-MB, LDH and cTnT at different time points. Meanwhile, the content of CREG protein in serum was determined by Western blot and compared with the control group. Results: The serum levels of CREG protein in AMI group were higher than that in control group within 72 hours (P <0.05). Within 2h after chest pain, the level of CREG in serum of AMI group was significantly higher than that of control group at 2h, 4h and 6h (P <0.001). Within 2 hours after onset of chest pain, there was no significant difference in serum cTnT, CK, CK-MB and LDH levels between the two groups (P> 0.05). Conclusion: The expression of CREG in serum of patients with AMI is higher than that of cTNT and CK-MB in serum.