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AIM:To investigate the effect of quercetin supplementation on the myenteric neurons and glia in the cecum of diabetic rats.METHODS:Total preparations of the muscular tunic were prepared from the ceca of twenty-four rats divided into the following groups:control(C),control supplemented with quercetin(200 mg/kg quercetin body weight)(CQ),diabetic(D)and diabetic supplemented with quercetin(DQ).Immunohistochemical double staining technique was performed with HuC/D(general population)/nitric oxide synthase(nNOS),HuC-D/S-100and VIP.Density analysis of the general neuronal population HuC/D-IR,the nNOS-IR(nitrergic subpopulation)and the enteric glial cells(S-100)was performed,and the morphometry and the reduction in varicosity population(VIP-IR)in these populations were analyzed.RESULTS:Diabetes promoted a significant reduction(25%)in the neuronal density of the HuC/D-IR(general population)and the nNOS-IR(nitrergic subpopulation)compared with the C group.Diabetes also significantly increased the areas of neurons,glial cells and VIP-IR varicosities.Supplementation with quercetin in the DQ group prevented neuronal loss in the general population and increased its area(P<0.001)and the area of nitrergic subpopulation(P<0.001),when compared to C group.Quercetin induced a VIP-IR and glial cells areas(P<0.001)in DQ group when compared to C,CQ and D groups.CONCLUSION:In diabetes,quercetin exhibited a neuroprotective effect by maintaining the density of the general neuronal population but did not affect the density of the nNOS subpopulation.
AIM: To investigate the effect of quercetin supplementation on the myenteric neurons and glia in the cecum of diabetic rats. METHODS: Total preparations of the muscular tunic were prepared from the ceca of twenty-four rats divided into the following groups: control (C) , supplemented with quercetin (200 mg / kg quercetin body weight) (CQ), diabetic (D) and diabetic supplemented with quercetin (DQ). Immunohistochemical double staining technique was performed with HuC / D (general population) / nitric oxide synthase nNOS), HuC-D / S-100 and VIP. Sensitivity analysis of the general neuronal population HuC / D-IR, the nNOS-IR (nitrergic subpopulation) and the enteric glial cells (S-100) was performed, and the morphometry and The reduction in varicosity population (VIP-IR) in these populations were analyzed .RESULTS: Diabetes promoted a significant reduction (25%) in the neuronal density of the HuC / D-IR (general population) and the nNOS-IR (nitrergic subpopulation ) compared with the C group. Diabetes also significantly increased t he areas of neurons, glial cells and VIP-IR varicosities. Skip composition with quercetin in the DQ group prevented neuronal loss in the general population and increased its area (P <0.001) and the area of nitrergic subpopulation (P <0.001), when compared to C group. Quercetin induced a VIP-IR and glial cells areas (P <0.001) in DQ group when compared to C, CQ and D groups. CONCLUSION: In diabetes, quercetin exhibited a neuroprotective effect by maintaining the density of the general neuronal population but did not affect the density of the nNOS subpopulation.