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A solid phase radioimmunoassay for free triiodothyronine in serum was developed based on double-antibody coated tubes. The method was turned out to be reliable with good reproducibility, higher sensitivity and easy performance. The measurable range of FT_3 in serum was 1.2 to 38 pmol/L. The mean coefficients of variation within and between assays were 1.79%-3.18% and 4.72%-9.31%, respectively. The FT_3 concentrations in euthyroid serum as determined by this method were 2.8 to 7.8pmol/L. The FT_3 values determined by this new method correlated well with those measured by a commercial radioimmunoassay (r=0.853).
A solid phase radioimmunoassay for free triiodothyronine in serum was developed based on double-antibody coated tubes. The method was turned out to be reliable with good reproducibility, higher sensitivity and easy performance. The measurable range of FT_3 in serum was 1.2 to 38 pmol / L. The mean coefficients of variation within and between assays were 1.79% -3.18% and 4.72% -9.31%, respectively. The FT_3 concentrations in euthyroid serum as determined by this method were 2.8 to 7.8 pmol / L. The FT_3 values determined by this new method correlated well with those measured by a commercial radioimmunoassay (r = 0.853).