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目的探讨驱铅益智口服液对染铅大鼠学习记忆障碍的改善作用。方法72只SD大鼠随机分为6组:阴性对照组、模型对照组、乙二胺四乙酸二钠盐(EDTA)组和低、中、高剂量口服液治疗组;除阴性对照组外,其余5组用2%醋酸铅灌胃3周造模;EDTA组腹腔注射0.5%EDTA-Na2,低、中、高剂量组分别灌胃给予1 250,2 500和5 000 mg/(kg.bw)驱铅益智口服液,连续3周;采用避暗穿梭试验和Morris水迷宫试验,观察各组染铅大鼠学习记忆行为,采用氢化物发生-原子吸收光谱法测定血液和海马组织铅含量。结果与模型对照组比较,中、高剂量组大鼠主动回避正确率(IAP)分别为68.1%和70.3%,明显高于模型对照组的54.9%(P<0.05);被动回避潜伏期(LOPA)分别为16.44和13.88 s,明显短于模型对照组27.43 s(P<0.01);口服液组血液和海马组织铅含量低于模型对照组(P<0.05或P<0.01);LOPA与海马组织铅含量呈正相关(r=0.475,P=0.014);IAP与海马组织铅含量呈负相关(r=-0.353,P=0.002)。结论驱铅益智口服液可通过减低血液与海马组织铅浓度改善学习和记忆功能。
Objective To investigate the effect of Zhaogan Yizhi oral liquid on learning and memory impairment of lead-exposed rats. Methods 72 SD rats were randomly divided into 6 groups: negative control group, model control group, ethylenediaminetetraacetic acid disodium salt (EDTA) group and low, medium and high dose oral liquid treatment group; in addition to the negative control group, The other 5 groups were treated with 2% lead acetate for 3 weeks. The rats in EDTA group were intraperitoneally injected with 0.5% EDTA-Na2. The low, medium and high dose groups were administered with 1 250, 2 500 and 5 000 mg / (kg.bw) Lead Yizhi Oral Liquid for 3 weeks. The learning and memory behaviors of the lead-exposed rats were observed by dark-avoidance shuttle test and Morris water maze test. The contents of lead in blood and hippocampus were determined by hydride generation-atomic absorption spectrometry. Results Compared with the model control group, the IAP of middle and high dose group was 68.1% and 70.3% respectively, which was significantly higher than that of the model control group (54.9%, P <0.05). The passive avoidance latency (LOPA) (P <0.05 or P <0.01). The content of lead in blood and hippocampus of oral solution group was significantly lower than that of model control group (P <0.05 or P <0.01). LOPA and lead (R = 0.475, P = 0.014). There was a negative correlation between IAP and lead in hippocampus (r = -0.353, P = 0.002). Conclusions CDFZ can improve learning and memory function by reducing lead concentration in blood and hippocampus.