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用“中国1号小型猪”经口感染HP30天后取食管、胃体、胃窦、十二指肠、小肠、大肠标本行尿素酶试验、W-S银染色、HP培养、HP-PCR等检查。在15只猪中除对照组2只外,其余13只均有HP感染,胃窦HP阳性率最高,为12/13,小肠HP阳性率最低,为0/13。尿素酶试验的敏感性、特异性为88.1％、85.7％;W-S银染色的敏感性、特异性为57.l％、100％;HP培养的敏感性、特异性为31.0％、100％;HP-PCR的敏感性、特异性为100％、83.3％。提示本实验所用检测HP感染方法,其敏感性和特异性可相互补充,应同时应用。取材部位对诊断猪HP感染影响较大,应多部位取材。
The tumors of the esophagus, gastric body, gastric antrum, duodenum, small intestine and large intestine were inoculated with “China No.1 Minipig” for 30 days. The urease test, W-S silver staining, HP culture and HP-PCR were performed. In 15 pigs, except for the control group 2, the other 13 had HP infection. The highest positive rate of gastric antrum was 12/13, and the lowest positive rate of intestinal HP was 0/13. The sensitivity and specificity of urease test were 88.1% and 85.7%, respectively. The sensitivity and specificity of WS silver staining were 57.1% and 100%, respectively. The sensitivity and specificity of HP culture were 31.0% and 100%, respectively. HP -PCR sensitivity, specificity of 100%, 83.3%. Tips used in this experiment detection of HP infection, the sensitivity and specificity can complement each other, should be applied at the same time. Drawn parts of the diagnosis of pig HP infection, should be more parts drawn.