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采用十种生物素化凝集素 U EA- I、 DBA、 PSA、 BSL、 PNA、 L CA、 RCA- I、SBA、 Con A及 WGA分别对 8W、1 4 W、 2 0 W 2 8W及 32 W的人胎儿食管上皮进行研究 ,以确定胎儿食管上皮在发育过程中凝集素的结合位点以及结合方式随年龄的变化。结果提示 ,BSL、 RCA- I、 WGA的染色强度及特性不受年龄影响 ,而与之相对应 ,U EA - 1在除 2 0 W以外的食管上皮显色 ;含α- D- Glc NAc的糖复合物 (DBA受体 )只在 2 0 W表达 ;含α- D- man/α- D- Glc的糖复合物 (PSA受体 )则只出现于 32 W;Con A受体虽在胎儿食管上皮各年龄组都表现阳性反应 ,但在 2 8W 时受体在上皮各层细胞中的分布方式及染色强度出现了有意义的变化 ,即腔面细胞染色阴性 ,中层细胞染色强阳性 ,基底细胞弱阳性 ,而在其他时间腔面反应强阳性 ,中层中等阳性 ,基底弱阳性 ;PNA、SBA及 L CA在各年龄组均呈阴性反应。这些结果表明 ,在胎儿食管上皮发育过程中存在着多个凝集素结合位点 ,含 α- L- Fucose、 α- D- Gal NAc、α- D- Man/ α- D- Glc残基的糖复合物在分布方式和 /或量上存在着发育相关性改变 ,即糖复合物的改变具有阶段性。总之 ,本实验资料提示 ,胎儿食管上皮在发育过程中表现出不同的结合位点 ,RCA- 、 BSL、 WGA受体似与年龄变
Ten kinds of biotinylated lectins U EA-I, DBA, PSA, BSL, PNA, L CA, RCA-I, SBA, Con A and WGA were used for the analysis of 8W, 14 W, 20 W 2 8W and 32 W Of human fetal esophageal epithelial research to determine the developmental process of fetal esophageal lectin binding sites and binding changes with age. The results suggested that the staining intensity and characteristics of BSA, RCA - I and WGA were not influenced by age, but correspondingly, U EA - 1 developed color in esophageal epithelium except 20 W; The glycoconjugate (DBA receptor) was expressed only at 20 W; the carbohydrate complex (PSA receptor) with α-D-man / α-D-Glc appeared only at 32 W; Esophageal epithelium showed positive reaction in all age groups, but there was a significant change in the distribution and staining intensity of the receptor in the epithelial cells at 28 days, ie, the staining of the luminal cells was negative, the staining of the middle cells was strongly positive, The cells were weakly positive, while at other times the surface reaction was strongly positive, the middle medium was positive and the basal was weakly positive. PNA, SBA and L CA were negative in all age groups. These results indicate that there are multiple lectin binding sites, including sugars of α-L-Fucose, α-D-GalNAc, α-D- Man / α-D-Glc residues, during fetal esophageal epithelial development There is a development-related change in the distribution pattern and / or amount of the complex, that is, the change of the sugar complex is stepwise. In conclusion, the experimental data suggest that fetus esophageal epithelium showed different binding sites during development, RCA-, BSL, WGA receptors and age-related changes