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将高浓度大肠杆菌Ｏ６的ＨＡＭ’ｓＦ１０悬液置入３７℃恒温孵化１０、３０和１８０ｍｉｎ后，分别稀释、过滤和离心制备大肠杆菌Ｏ６悬液、滤液和离心上清液，然后将其在体外与１０例健康成年生育男性精子一起孵化０、１、２、４和６ｈ，再采用德国Ｍｉｋａ计算机辅助的精子分析系统分析大肠杆菌Ｏ６悬液及其滤液和离心上清液体对精子前向运动百分率的影响。结果显示：不同孵化时间的大肠杆菌悬液在体外均能引起人精子前向运动百分率显著降低，但其滤液和离心上清液无类似作用。因此，可以认为大肠杆菌Ｏ６在体外对精子的作用在于细菌本身，而细菌毒素并无作用。
HAM’sF10 suspension of high concentration Escherichia coli O6 was incubated at 37 ° C. for 10, 30 and 180 min. After dilution, filtration and centrifugation respectively, the Escherichia coli O6 suspension, the filtrate and the supernatant were prepared and then cultured in vitro 10, 1, 2, 4, and 6 h were incubated with 10 healthy adult male spermatozoa, and the percentage of forward movement of sperm was analyzed by using Mika computer-assisted sperm analysis system of Germany Mika E. coli O6 suspension and its filtrate and centrifuged supernatant liquid Impact. The results showed that: Escherichia coli suspensions with different hatching time could significantly reduce the percentage of human sperm forward movement in vitro, but the filtrate and centrifugation supernatant had no similar effect. Therefore, Escherichia coli O6 can be considered in vitro role of sperm in the bacteria themselves, and bacterial toxins have no effect.