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目的研究海洋生物海燕(Asterina pectinifera)的化学成分。方法采用大孔树脂柱色谱、硅胶柱色谱、凝胶柱色谱、制备薄层色谱、高效液相色谱等手段分离纯化,根据理化性质、波谱分析和文献对照的方法对所分离的化合物进行结构鉴定。结果从海燕体内分离并鉴定了9个化合物,分别为:3-十八烷氧基-1,2-丙二醇[3-(octadecyloxy)-1,2-propanediol,1]、正二十碳酸(arachidic acid,2)、胆甾-7-烯-3β-醇(cholesta-7-ene-3β-ol,3)、尿嘧啶(uracil,4)、5α-胆甾烷-3β,4β,6α,7α,8,15β,16β,26-八醇(5α-cholestane-3β,4β,6α,7α,8,15β,16β,26-octol,5)、5α-胆甾烷-3β,6α,8,15α,24-五醇(5α-cholestane-3β,6α,8,15β,24-pentaol,6)、色氨酸(tryptophan,7)、3,4-二羟基苯甲酸(3,4-dihydroxybenzoic acid,8)、pectinioside B([(5α,20R,22R,23S,24S)-22,23-epoxy-3β,6α,20-trihydroxyergost-(9)11-ene 6-O-{β-D-galactopyranosyl-(1→4)-[β-D-quinovopyranosyl-(1→2)]-β-D-glucopyranosyl-(1→4)-[β-D-quinovopyranosyl-(1→2)]-β-D-xylopyranosyl-(1→3)-β-D-quinovopyranoside}-3-O-sodium sulfate],9)。结论化合物2、8为首次从海燕中分离得到,化合物9为首次从中国海域的海星纲中分离得到。首次采用二维核磁技术确定化合物9的结构,并首次对其1H-NMR数据及二维谱数据进行归属。
Objective To study the chemical composition of Asterina pectinifera. Methods The compounds were separated and purified by macroporous resin column chromatography, silica gel column chromatography, gel column chromatography, preparative thin layer chromatography and high performance liquid chromatography. The structures of the isolated compounds were identified by physicochemical properties, spectral analysis and literature comparison . Results Nine compounds were isolated and identified from the body of the petrel, which were 3-octadecyloxy-1,2-propanediol (1), arachidic (2), cholesta-7-ene-3β-ol (3), uracil (4), 5α-cholestan-3β, 4β, 6α, , 8,15β, 16β, 26-octol, 5α-cholestane-3β, 4α, 6α, 7α, 8,15β, 16β, , 24-pentaol (6α, 8,15β, 24-pentaol, tryptophan, 3,4-dihydroxybenzoic acid 8), pectinoside B ([(5α, 20R, 22R, 23S, 24S) -22,23 -epoxy-3β, 6α, 20-trihydroxyergost- (9) 11- ene 6-O- {β-D-galactopyranosyl- (1- → 4) - [β-D-quinovopyranosyl- (1 → 2)] - β-D-glucopyranosyl- xylopyranosyl- (1 → 3) -β-D-quinovopyranoside} -3-O-sodium sulfate], 9). Conclusion Compound 2 and 8 were isolated from sea petal for the first time. Compound 9 was isolated from sea star class in China for the first time. For the first time, the structure of compound 9 was confirmed by two-dimensional nuclear magnetic resonance and the 1H-NMR data and two-dimensional spectral data were assigned for the first time.