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以甜菜‘KWS0143’和‘Beta464’两个品种为试验材料,研究不同浓度(0、0.4%、0.8%和1.2%)Na2CO3胁迫下,甜菜苗期植株生长,叶片及根系的超氧化物歧化酶(SOD)、过氧化物酶(POD)及丙二醛(MDA)的变化。结果表明,同一取样时期,随着Na2CO3浓度的增加,植株的干鲜重呈先增加后降低的趋势,0.4%Na2CO3处理下甜菜幼苗干鲜重显著高于对照(P<0.05)。叶片和根中的SOD和POD活性变化趋势相一致,总体呈先升高后下降的趋势,在0.4%Na2CO3处理下活性最高。MDA含量则呈递增的趋势,1.2%Na2CO3处理下含量最多。同一浓度下,SOD和POD活性随着处理时间的延长呈先上升后下降的趋势,且在2对真叶展开后的第3周活性最高,而MDA含量则呈现急剧增加后缓慢上升的趋势。而且高浓度1.2%Na2CO3处理下‘KWS0143’耐盐碱能力强于‘Beta464’。
The effects of different concentrations (0, 0.4%, 0.8% and 1.2%) of Na2CO3 on the plant growth, leaf and root superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA) changes. The results showed that with the increase of Na2CO3 concentration, the fresh weight of the plant first increased and then decreased. The dry weight of the sugar beet seedling under 0.4% Na2CO3 treatment was significantly higher than that of the control (P <0.05). The trend of SOD and POD activity in leaves and roots was the same, and the trend was firstly increased then decreased, and the activity was the highest under 0.4% Na2CO3 treatment. MDA content showed an increasing trend, the highest content of 1.2% Na2CO3 treatment. At the same concentration, the activities of SOD and POD increased firstly and then decreased with the prolongation of treatment time, and reached the highest level in the third week after the development of two true leaves, while the MDA content showed a sharp increase and then increased slowly. Moreover, ’KWS0143’ was more resistant to salt and alkali than ’Beta464’ under high concentration of 1.2% Na2CO3.