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[目的]观察锰对大鼠脑谷氨酸代谢的影响。[方法]Wistar大鼠24只,按体重随机分成4组,每组6只。第1组为对照组,皮下注射0.9%氯化钠,第2～4组为锰染毒组,分别皮下注射8、40、200μmol/kg的氯化锰溶液,注射容量均为5mL/kg,染毒4周,每周5次。测定脑纹状体谷氨酰胺酶(PAG)、谷氨酰胺合成酶(GS)活性和谷氨酸(Glu)、谷氨酰胺(Gln)含量以及细胞凋亡率。[结果]染锰4周后,随着染锰剂量的增加,PAG活性逐渐升高,而GS活性逐渐下降;同时Glu含量也逐渐升高,Gln含量逐渐下降;脑纹状体细胞凋亡率明显升高(P<0.01)。[结论]锰可以通过增强PAG活性和抑制GS活性,使Glu-Gln循环代谢紊乱,产生神经毒性。
[Objective] To observe the effect of manganese on glutamate metabolism in rat brain. [Methods] Twenty-four Wistar rats were randomly divided into 4 groups according to body weight, with 6 rats in each group. The first group was the control group, subcutaneous injection of 0.9% sodium chloride, the second to the fourth group for the manganese exposure group, subcutaneous injection of 8,40,200μmol / kg of manganese chloride solution, the injection capacity of 5mL / kg, 4 weeks, 5 times a week. The glutathione peroxidase (PAG), glutamine synthetase (GS) activity, glutamate (Glu), glutamine (Gln) content and apoptosis rate were measured. [Result] With the increase of manganese dose, the activity of PAG gradually increased and the activity of GS gradually decreased. At the same time, the content of Glu gradually increased and the content of Gln decreased gradually. The apoptosis rate of brain striatum Was significantly higher (P <0.01). [Conclusion] Manganese can cause disorder of Glu-Gln cycle metabolism and produce neurotoxicity by enhancing PAG activity and inhibiting GS activity.