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经前期摇瓶试验筛选获得一种能够提高林可霉素发酵水、平的复合蛋白粉,使摇瓶发酵单位提高至4500μg/ml。在200 L发酵罐放大试验过程中,结合中间补料,考察了氮源对林可霉素产生菌菌丝生长及产物合成的影响。结果表明,含有复合生白粉的培养基中,溶解磷浓度显著高于对照组和其他试验组,显著延缓菌丝自溶,使林可霉素发酵单位提高至9561μg/ml。添加相相同浓度无机磷的培养基试验组虽然同样能够延缓菌丝的自溶和促进林可霉素的合成,但其提高能力远低于含复合蛋白粉培养基组。推测复合蛋白粉中的有机磷源可能在林可霉素的,生物合成过程中起重要作用。
Pre-shake shake flask screening test to obtain a can improve lincomycin fermentation water, flat composite protein powder, shake flask fermentation unit increased to 4500μg / ml. In the 200 L fermenter amplification test, combined with the intermediate feed, inspected the nitrogen source of lincomycin-producing bacteria mycelial growth and product synthesis. The results showed that the concentration of dissolved phosphorus was significantly higher than that of the control and other experimental groups in the culture medium containing compound raw meal, which significantly delayed the mycelial autolysis and increased the lincomycin fermentation unit to 9561 μg / ml. The medium supplemented with inorganic phosphorous at the same concentration could improve the ability of lincomycin much lower than that of the composite protein powder medium although it could delay the autolysis of mycelium and promote the synthesis of lincomycin. It is speculated that the organic phosphorus source in the composite protein powder may play an important role in the process of lincomycin biosynthesis.