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为了协同人白细胞介素-2（IL－2）与乙型肝炎（乙肝）病毒（HBV）前S抗原（preSAg）的生物学功能，探索能打破持续性感染者对HBV表面抗原（HBsAg）的免疫耐受，诱导机体对HB－sAg和preSAg产生体液和细胞免疫应答的基因免疫与治疗药物，用基因重组方法构建了IL－2和HBV完整preSAg的真核表达嵌合基因及表达质粒pCWIIP。pCWIIP转染的Cos－7细胞能分泌表达IL－2－preS融合蛋白，其表达效率与pCIIL－2转染的细胞表达IL－2一致，细胞上清中IL－2活性单位大于3×103u／ml，并用酶免疫实验法（EIA）检测了preSAg，而其细胞裂解液中IL－2活性很低，preSAg未检测到。本文结果说明preSAg2～19位氨基酸序列的滞留效应是可以克服的。该发现不仅为构建带完整preSAg的新型乙肝疫苗和特异性免疫治疗剂提供了理论与实验依据，而且对蛋白质分泌表达调控的研究也有一定意义。
To explore the biological function of interleukin-2 (IL-2) and pre-S antigen of hepatitis B virus (HBV), we explored the role of IL-2 in preeclampsia against HBV surface antigen (HBsAg) Immune tolerance and induction of humoral and cellular immune responses to HB-sAg and preSAg. The eukaryotic expression chimeric gene and expression plasmid pCWIIP of IL-2 and HBV intact preSAg were constructed by genetic recombination. The expression of IL-2-preS fusion protein was secreted by pCWIIP-transfected Cos-7 cells, which was consistent with the expression of IL-2 in pCIIL-2 transfected cells. The activity of IL-2 in the supernatant was higher than 3 × 103u / ml, and preSAg was detected by enzyme immunoassay (EIA). However, the activity of IL-2 in cell lysate was very low and preSAg was not detected. Our results demonstrate that the retention of the amino acid sequence of amino acids 2-9 of preSAg can be overcome. This finding not only provides a theoretical and experimental basis for the construction of new hepatitis B vaccine and specific immunotherapeutics with intact preSAg, but also has some significance for the study of the regulation of protein secretion and expression.