论文部分内容阅读
目的：观察和分析细胞程序性死亡（ＰＣＤ）在细胞凝聚区形成中的作用和意义。方法：建立和制备Ｃ５７ＢＬ／６Ｎ系小鼠胚胎期１３、１４、１５、１６ｄ和生后１０ｄ等多时间点的颌面部发育模型，采用原位末端转移酶标记法标记颌面部组织的ＰＣＤ细胞。结果：在颌面部骨、牙齿等多种组织器官形成初期都包括间充质细胞的聚集，并出现特定的细胞凝聚区。随着细胞凝聚的出现，ＰＣＤ的数量和密度增多。当凝聚区内的细胞向特定细胞系表型转化时，ＰＣＤ数量下降。结论：ＰＣＤ在颌面部凝聚区的形成中发挥着重要的作用，可控制其中的细胞数量和质量并有利于凝聚区的塑形
Objective: To observe and analyze the role and significance of programmed cell death (PCD) in the formation of cellular aggregates. Methods: The maxillofacial development model of C57BL / 6N mouse embryo at 13, 14, 15, 16d and 10d after birth was established and prepared. The PCD of maxillofacial tissue was detected by in situ terminal transaminase cell. Results: In the early stages of the formation of various tissues and organs such as maxillofacial bone and teeth, the mesenchymal cells were aggregated and specific cell aggregation areas appeared. With the appearance of cell aggregation, the number and density of PCD increased. The number of PCDs decreased when the cells in the cohesion transformed to a specific cell line phenotype. Conclusions: PCD plays an important role in the formation of maxillofacial condensation zone, which can control the number and quality of cells therein and facilitate the shaping of cohesive zone