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离体小麦叶片在暗处叶绿素迅速分解，过氧化物酶活性升高，精胺处理可以延缓叶绿素降解并且降低过氧化物酶的活性。过氧化物酶同工酶的聚丙烯酸胺凝胶电泳结果显示，衰老叶片除保持原有的３条同工酶活性外，还出现１条新的同工酶，该酶活性随时间延长而上升。精胺处理则一直无此酶的出现。另外，随精胺处理时间的延长，有两条同工酶活性不断降低，另一条基本保持不变。根据过氧化物酶在植物体中的作用，推测精胺保绿作用的机理与精胺抑制过氧化物酶活性或降低过氧化物酶活性有关。
In vitro chlorophyll rapidly decomposed and the activity of peroxidase increased in the dark. Spermine treatment delayed the chlorophyll degradation and decreased the activity of peroxidase. Polyacrylamide gel electrophoresis of peroxidase isozymes showed that in addition to maintaining the original three isozymes, aging leaves also appeared a new isozyme, the enzyme activity increased with time . Spermine treatment has been the absence of this enzyme. In addition, with the extension of spermine processing time, there are two isozyme activity decreased, the other remained unchanged. According to the role of peroxidase in plants, it is speculated that the mechanism of spermine greening is related to inhibition of peroxidase activity or peroxidase activity by spermine.