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目的:探讨大鼠视网膜细胞缺血再灌注后低氧诱导因子(HIF-1α)的表达、视网膜细胞凋亡的发生以及二者之间的关系。方法:采用前房灌注生理盐水升高大鼠眼压到110mmHg(1kPa=7.5mmHg)持续1h的方法制作实验性视网膜缺血再灌注损伤模型,分别于再灌注不同时间点取材,行免疫组织化学法染色观察HIF-1α在视网膜细胞的阳性表达;采用DNA原位末端标记(terminal dUTP nick end labelling,TUNEL)法定位凋亡的视网膜细胞来检测视网膜凋亡细胞。结果:缺血再灌注2h组视网膜神经节细胞层及内核层细胞出现HIF-1α弱阳性表达,12h组阳性表达至高峰,24h组HIF-1α表达渐减少。视网膜组织细胞凋亡见于缺血再灌注12,24及48h组,凋亡细胞主要位于内核层,且24h组凋亡阳性表达最强。结论:缺血再灌注后大鼠视网膜HIF-1α的表达增加,参与视网膜缺血再灌注损伤;视网膜细胞的损伤部分以凋亡的形式发生,HIF-1α的表达可能与视网膜细胞凋亡有密切的关系。
Objective: To investigate the expression of hypoxia-inducible factor (HIF-1α), retinal cell apoptosis and the relationship between them after retinal ischemia-reperfusion in rat retinal cells. Methods: The model of experimental retinal ischemia / reperfusion injury was established by anterior chamber perfusion with saline to increase the intraocular pressure of rats to 110mmHg (1kPa = 7.5mmHg) for 1h. The rats were drawn at different time points after reperfusion, and immunohistochemistry The positive expression of HIF-1α in retinal cells was observed by staining. The apoptosis of retinal cells was detected by using terminal dUTP nick end labeling (TUNEL). Results: The expression of HIF-1α was weakly positive in retinal ganglion cell layer and inner nuclear cell layer at 2h after ischemia-reperfusion, and peaked at 12h. The expression of HIF-1α in 24h group decreased gradually. Retinal tissue apoptosis was found in ischemia reperfusion group 12,24 and 48h, apoptotic cells located in the inner nuclear layer, and 24 hours apoptosis was the highest expression. Conclusion: The expression of HIF-1α in the retina of rats after ischemia-reperfusion is increased, which is involved in retinal ischemia-reperfusion injury. The damage part of retinal cells takes the form of apoptosis, and the expression of HIF-1α may be closely related with the apoptosis of retinal cells Relationship.