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The Ramsey fringes on the ground-state hyperfine transition of 199Hg+ (ΔF =1,ΔmF =0) ions trapped in a hyperbolic Paul trap are firstly observed with the method of time-separated oscillatory fields.The full width at half maximum of central Ramsey fringes is about 25 mHz and the corresponding quality factor Q of the line is greater than 1012 for the trapped 199Hg+ microwave frequency standard.The hyperfine transition frequency shifted by magnetic field is also measured by the high-resolution Ramsey fringes.The final result is ΔVhfs =40507347997.3(0.5) Hz,which is corrected to zero magnetic field.