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An atmospheric cold plasma brush suitable for large area and low-temperature plasma-based sterilization is designed and used to treat enterococcus faecalis bacteria.The results show that the efficiency of the inactivation process by helium plasma is dependent on applied power and exposure time.After plasma treatments,the cell structure and morphology changes can be observed by scanning electron microscopy.Optical emission measurements indicate that reactive species such as O and OH play a significant role in the sterilization process.