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目的:研制一种适合于肠道杆菌L型生长的培养基。方法:采用平板纸片法诱导肠道杆菌(伤寒、痢疾、大肠)及金黄色葡萄球菌为L型,观察它们在肠道杆菌L型培养基上的生长情况,并与改良Kagan培养基相对照。结果:在改良Kagan培养基上四种细菌L型均生长,而在肠道杆菌L型培养基上金黄色葡萄球菌及其L型不生长,肠道杆菌及其L型生长良好,且可区别出肠道致病菌及大肠杆菌。结论:肠道杆菌L型培养基适用于肠道标本细菌L型的分离。
Objective: To develop a suitable medium for the growth of intestinal bacilli L type. Methods: The enterobacteriaceae (typhoid, dysentery, large intestine) and Staphylococcus aureus were induced to form L form by the method of flat paper, and the growth of them was observed on the L type medium of enterobacteriaceae and compared with the modified Kagan medium . RESULTS: All four L-forms grew on modified Kagan medium, while Staphylococcus aureus and its L-form did not grow on enteric-bacilli L-medium, and intestinal Lactobacilli and their L-forms grew well and were distinguishable Enteric pathogens and E. coli. Conclusion: Enterobacter L-type medium is suitable for the isolation of bacterial L-forms in intestinal samples.